The Cytometry Platform uses immunophenotypic techniques to determine the characteristics of particles and cells in suspension, and which pass individually through the light emitted by lasers. The analysis and differentiation of cells and particles are based on their light-scattering properties (morphology and cell complexity) and on the emission of fluorescence due to the binding of specific monoclonal antibodies or fluorescent colorants.
Flow cytometers are equipped with two or more lasers as light sources, different optical configurations that combine filters and mirrors and separate the light emitted by the fluorochromes, electronic systems that help detect and amplify the signals detected, and a computer that transforms these signals into data. The fluorochromes that should be used are determined by the characteristics of the lasers and the optical configurations available in each cytometer.
Flow cytometry has many applications. It is now used extensively in the fields of medicine (hematology, solid tumors and immunology), cell biology, and cell separation for subsequent genomic and functional studies.