Processing Software and Image Analysis

The Imaging Platform offers software implementation, training and support for image processing and analysis at workstations.

Some of the methods available are listed below:
(On request, these and other methods can be custom-configured and optimized for the needs of the user.)

  • Collagen detection: Method for segmenting collagen in images of tissue stained with Sirius Red. Measurements are obtained, including the area occupied by the collagen, intensity values, area of precipitated porphyrins, etc. [Image]

  • Segmenting and counting positive nuclei: Example of application: The software developed can detect and count the number of proliferative cells of epithelial tissue in lung images, through immunolabeling with ki67 in tumors and preneoplastic lesions. [Image]

  • Adipocyte counting: Method for counting the cells in an image of fatty tissue, record size and create histograms with size distributions per sample from the results obtained. [Image]

  • Measurements of fluorescent label colocalization: Different methods (ICQ, Pearsons, Manders, Spearman, Intersection, Overlap coefficients) for quantifying the degree of overlap between two or more fluorochromes with different wavelengths. [Image] [Image]

  • Mouse skin image analysis: Method for detecting the labeling made by an antibody (violet color) inside a region of interest (ROI) in different samples of mouse skin. Once detected, statistical studies can be carried out on several measurements of the labeling. [Image]

  • Epithelial cell analysis: Method for counting and quantifying epithelial cells from membrane fluorescence labeling. Quantification provides the distribution of cells according to size and number of neighboring cells. [Image]

  • Wound healing studies: Method for analyzing images and videos in a wound healing study. Analyzes the area covered by the wound and the speed and acceleration of cell migration throughout the study. [Image] [Image]

  • Human Angiogenesis Array Analyzer: Method for quantifying arrays of points developed on X-ray film (with Proteome ProfilerTM Array). [Image]