Regulation of transcription is one of the most important parts of functional genomics. Analysis of gene-expression profiles, also called transcriptomics, has been carried out by our service since 2007 using GeneChip® arrays that employ Affymetrix technology specific to each genome.
The service currently processes the following routinely:
Gene ST Array. This array contains 26 probes per gene, designed to cover the whole gene. It can be used for complete differential expression studies, in which it is not necessary to detect splicing variants. Besides rat, mouse and human, versions exist for many other genomes (please inquire).
Transcriptome Array. A high-resolution array available for humans and mice: It contains more than 6 million probes for coding and non-coding transcripts. To ensure uniform results, it has been designed with 10 probes per exon and 4 for each alternative splicing region.
miRNA Array. An array designed to study microRNA. It is provided as a tool for studying the role of small non-coding RNA molecules (miRNA, snoRNA and scaRNA) in cancer and other diseases.
CytoScan HD Array. The CytoScan array is the molecular cytogenetics solution of Affymetrix technology, which makes it possible to detect human chromosomal aberrations. It includes 750,000 SNPs, which make it possible to detect, with more than 99% accuracy, loss of heterozygosity (LOH), CNVs, maternal contamination, low-level mosaicism, etc. It is also capable of providing information on the heterogeneity of the sample, clonal diversity and uniparental disomy (UPD).