FACSARIA-IIU (BD BIOSCIENCES)
This high-speed digital cell sorter separates up to four cell populations simultaneously. It is equipped with four laser lines: blue (488 nm), red (633 nm), violet (407 nm) and near-violet (375 nm). Up to three can be used at the same time. It has sensors and filters organized in three optical configurations with different lasers, mirrors, filters and sensors that detect a total of 11 fluorescence parameters. Moreover, different pressures can be used depending on the size and fragility of the cells to be sorted. It can separate cells into different containers, including 5-ml and 15-ml tubes and plates with 96 wells. It also has a unit that sorts one cell per well, which is very useful for cloning. It uses FACS Diva v6.1.3 acquisition software.
This cytometer is only handled by the personnel in the Flow Cytometry Service. Users must make a reservation in advance for its use (firstname.lastname@example.org).
FACSCANTO (BD BIOSCIENCES)
This instrument is a flow cytometer for analytical cytometry equipped with three lasers (blue: 488 nm; red: 633 nm; and violet: 405 nm) organized into two optical configurations with mirrors and filters to analyze up to eight fluorescences. It is able to acquire up to 10,000 events per second, requires less sample volume and has a sensitivity capable of resolving events with weaker signals. Carryover between samples is minimal (<0.1%). As with the FACAria, it uses FACS Diva v6.1.3 acquisition software. This cytometer is operated by the researchers themselves, so they must first receive mandatory instructions from the Flow Cytometry Service.
Furthermore, CIMA's Flow Cytometry Service provides users with two licenses for the FACS Diva v6.1.3 program, so they can analyze the data acquired using FACSAria and FACSCanto.