Bioinformatics Service

Its main goal is to contribute to the development of the research projects carried out at CIMA, for which it provides complete support in the development of any experiment involving large-scale analysis of DNA or RNA of humans and other organisms. The tasks carried out include:

  • Experimental design. Guidance on deciding the sample size, study design and method planning.

  • Data analysis. Bioinformatics processing is carried out on the data obtained using different high-performance technological platforms employed in the area of genomics and proteomics (microarrays, sequencing, PCR, etc.). These analyses include standardization, statistical analysis and representation of the results.

The most common analyses include:

  1. Differential gene expression, miRNAs and lncRNAs. The source technology of the experiments may be microarrays (Affymetrix, Agilent), large-scale PCRs (TLDAs from Applied Biosystems) or RNA-seq (Illumina, Ion Torrent).

  2. Studies on genotyping and DNA copy number variation with microarrays (Affymetrix) or DNA sequencing (Illumina).

  3. Characterization of alternative splicing from the data obtained in microarray experiments (Affymetrix) or RNA-seq (Illumina).

  4. Protein-DNA interactions from experiments of immunoprecipitation and microarrays (ChIP-chip) or sequencing (ChIP-seq).

  5. DNA methylation patterns in experiments with microarrays (Illumina Infinium).

More recently, shotgun and semi-quantitative high-resolution proteomics data analysis protocols have been developed.

  • Assistance in the interpretation of results. Once the relevant genes are selected, studies on biological functional assignment are carried out. This type of analyses can be very broad and may vary depending on the specific goals of each study. 

"Our facility has the necessary goal of technological innovation, which is achieved in the context of national and international projects", Dr. Víctor Segura, Bioinformatician.

Contact

Contact:
Cristina López
Avda. Pío XII, 53
31008 Pamplona
Spain

(+34) 948 194 700 Ext. 6021
clopezg@unav.es